Project Information

Reference Number 19910001
Project Title Transformation in Indica Rice
Project Type Contained Use (Laboratory)
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution Tokyo University
Supervising IBC IRRI-IBC
Project Leader(s) Francisco J. Zapata, Tissue Culture Specialist, IRRI
Experimental Facility/Site Philippines (IRRI) and Japan (Tokyo University)
Purpose / Objectives 1. To develop efficient techniques for the establishment of cell suspension and protoplast culture of indica rices.
2. To transfer the gene of interest, initially into japonica and eventually into indica rices using different methods of delivery.
3. To analyze the F1s of presumptive transformants for gene expression.
Biosafety measures 1. The regenerated plants will be grown in the phytotron or containment facility.
2. Vegetative materials will be incinerated and the seeds will be kept in sealed cans for storage.
Conditions for Approval Should comply with the guidelines endorsed by NCBP
Date Received 1991-07-24
Date of Approval 1991-09-26


Regulated Materials

Regulated Materials 1 ug GUS Plasmid (PB1221)
Source University of Tokyo, Japan
Subject Request to import GUS plasmid (pBI 221)
Requesting Party Dr. Francisco J. Zapata, Tissue Culture Specialist,IRRI
Action of NCBP Approved for implementation;
For issuance of permit to import 1 ug of GUS Plasmid (PB1221) by the Plant Quarantine Services, Bureau of Plant Industry