Project Information

Reference Number 20000140
Project Title Marker-Aided Transfer of Bacterial Leaf Blight and Rice Tungro Disease Resistance Genes from Transgenic Lines to Recommended Varieties of Rice
Project Type Contained Use (Glasshouse)
Status Completed
Name of Institution Philippine Rice Research Institute (PhilRice),Maligaya, Science City of Muñoz, Nueva Ecija,(044) 456-0426/0415 ext. 259
Cooperating Institution Asian Rice Biotechnology Network (ARBN)
Supervising IBC PhilRice-IBC
Project Leader(s) Leocadio Sebastian and Dindo Tabanao, Executive Director
Experimental Facility/Site PhilRice, Munoz, Nueva Ecija, Philippines
Purpose / Objectives To employ molecular markers in transferring BLB and RTSV resistance genes from transgenic materials to recommended Philippine varieties
Biosafety measures 1. Transgenic plants will be kept in isolation in the glasshouse which is locked all the time to prevent entry of unauthorized personnel.
2. Test plants will be grown in mylar plastic cages for inoculation and to be enclosed in larger cages covered with nylon- mesh screens at the later growth stages inside the screenhouse.
3. Only male N. virescens will be used for inoculation. After inoculation, the insects will be collected and destroyed.
4. Panicles of flowering plants will be bagged before anthesis to prevent pollen dispersal and contamination.
5. Leaf samples of test plants will be placed in sealed plastic bags inside an ice chest when transporting to the laboratory for ELISA and PCR.
6. Test plants will be incinerated and buried upon completion of phenotyping of termination of experiment.
7. Used soil will be sterilized upon termination of the experiments.
8. Harvested seeds from transgenic plants will be sealed in the plastic bags, properly labeled, and kept in a sealed container, in isolation from other seeds.
9. Harvested seeds from transgenic plants will be disposed properly (by autoclaving before disposing) when no longer needed.
Conditions for Approval N/A
Date Received 2000-10-24
Date of Approval 2001-01-09


Regulated Materials

Regulated Materials N/A
Source N/A
Subject Request of Dr. Rodante Tabien for proposal endorsement
Requesting Party Dr. Rodante Tabien, Chairman, PHILRICE- IBC
Action of NCBP Approved for implementation