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Project Information


Reference Number 20020154
Project Title Development of DNA- Based Detection Procedures for Genetically Modified Plants
Project Type Contained Use
Status Completed
Name of Institution National Institute of Molecular Biology and Biotechnology (NIMBB), UP Diliman, Quezon City
Cooperating Institution none
Supervising IBC UP Diliman-IBC
Project Leader(s) Cynthia Hedreyda, Associate Professor
Experimental Facility/Site NIMBB-UP Diliman, Philippines
Purpose / Objectives 1. To perform extensive literature search on modified genes that have been introduced into plants (GMO) that the Philippines imports (or would possibly import) as seeds and seedlings.
2. To optimize DNA extraction from seeds and seedlings of corn and soybean.
3. To design PCR primers for the amplification of introduced genes and to optimize parameters for PCR.
4. To develop a quick PCR- based and non- radioactive DNA hybridization procedures for the routine detection and monitoring of genetically modified genes.
Biosafety measures 1. E. coli JM 105 and/or DH5 cells will be handled inside class II biological safety cabinet.
2. Equipment, glassware, and other materials to be used in handling bacteria will be properly rinsed with bleach and detergent, and sterilized by autoclaving.
3. Materials to be disposed of will also be decontaminated by autoclaving prior to disposal.
Conditions for Approval N/A
Date Received 2002-04-05
Date of Approval 2002-04-23

 


Regulated Materials


Regulated Materials none
Source N/A
Subject Request of Dr. Cynthia Hedreyda for acquiring non- viable samples of transgenic corn and soybeans
Requesting Party Dr. Cynthia Hedreyda, Associate Professor
Action of NCBP Approved for implementation