|Project Title||Agrobacterium-mediated Rice Tungro Bacilliform Virus Transfer System for Enhancing Resistance to Rice Tungro Disease|
|Project Type||Contained Use|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Cooperating Institution||John Innes Center, Norwich, UK;
University of Delhi, New Delhi, India;
University of Nebraska, USA;
|Project Leader(s)||Il-Ryong Choi, Plant Virologist|
|Experimental Facility/Site||IRRI, Laguna, Philippines|
|Purpose / Objectives||1. To import strains of Agrobacterium and relevant DNA plasmids to establish an insect-free inoculation system for Philippine strains of rice tungro bacilliform virus (RTBV).
2. To characterize Philippine indigenous isolates of RTBV that are potentially threatening to rice production.
3. To identify DNA sequences in the genome of RTBV which determine the severity of rice tungro disease (RTD).
4. To monitor the temporal changes of the RTBV genome in rice fields to prolong the life of virus- resistant varieties.
5. To prepare pure RTBV samples for the production of high quality RTBV antibody indispensable for reliable assays of RTBV infection.
|Biosafety measures||1. Only authorized personnel who are fully aware of the biosafety guidelines will perform DNA manipulation in the laboratories located in the EPPD Annex.
2.Inoculated plants will be kept in Bay 8 of the CL4 facility and only the authorized personnel will handle the experiments.
3. All biological waste from the laboratories and plant materials from the CL4 facility will be placed in plastic bags and autoclaved or incinerated before disposed.
4. Glassware, pots and soil will be autoclaved or baked after each use.
|Conditions for Approval||N/A|
|Date of Approval||2003-07-21|
|Regulated Materials||1. 1 g Agrobacterium tumefaciens strain GV 3850; 2. 1 g Agrobacterium tumefaciens strain GV 3111, 1 g pMON521 (in E. coli), 1 g pRK2013 (in E. coli); 3. pBIN19 (in E. coli)|
|Source||1. John Innes Center, Norwich, UK; 2. University of Nebraska, USA; 3. University of Delhi, New Delhi, India|
|Subject||Recommendation of Atty. Blo Umpar Adiong for permit to import pBIN19 and Agrobacterium tumefaciens strain GV 3850|
|Requesting Party||Atty. Blo Umpar Adiong, Director, Bureau of Plant Industry|
|Action of NCBP||Approved for implementation
For issuance of permit to import pBIN19 and Agrobacterium tumefaciens strain GV 3850 by the Plant Quarantine Services, Bureau of Plant Industry