Project Information

Reference Number 20030170
Project Title Improvement of Bacillus thuringiensis Cry Protein Function by Directed Gene Evolution
Project Type Contained Use (Laboratory)
Status Terminated
Name of Institution National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Banos, College, Laguna,049-536-2721; 536-2724
Cooperating Institution none
Supervising IBC UPLB-IBC
Project Leader(s) Edwin Alcantara, University Researcher I
Experimental Facility/Site BIOTECH-UPLB, Laguna, Philippines
Purpose / Objectives To create new Cry proteins with improved insecticidal activity to diamondback moth Plutella xylostella.
Biosafety measures 1. Imported and locally transformed E. coli harboring cloned cry genes will be stored in glycerol at –80° C
2. All aseptic work will be done in a laminar flow hood.
3. Growing of recombinant E.coli cells will be done in enclosed shakers with properly regulated speed and temperature.
4. All glasswares and solid and liquid wastes coming from the production of Cry proteins will be sterilized at 121° C for 20 mins. In an autoclave.
Conditions for Approval No viable resistant insect (particularly Plutella xylostella) will be brought to Los Baños or to any other areas where resistance does not exist and compliance with all biosafety measures indicated in the proposal.
Date Received 2003-06-04
Date of Approval 2003-07-17


Regulated Materials

Regulated Materials cry genes (cry1C, cry1Ac)
Source Bacillus Genetic Stock Center, Columbus, Ohio, USA
Subject Request of Dr. Marina Natural for permit to import cry genes (cry1C, cry1Ac)
Requesting Party Dr. Marina Natural, Chairman, UPLB-IBC
Action of NCBP Approved for implementation

For issuance of permit to import cry genes (cry1C, cry1Ac) by the Plant Quarantine Services, Bureau of Plant Industry