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Project Information


Reference Number 19930016
Project Title Applications on Improvement of Biolistic Transformation Methods (Addendum I)
Project Type Contained Use (Laboratory)
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution Division of Plant Industry, CSIRO, Australia
Supervising IBC IRRI- IBC
Project Leader(s) John Bennett, Senior Molecular Biologist
Experimental Facility/Site Philippines (IRRI)
Purpose / Objectives To be used for rice transformation studies.
Biosafety measures 1. The transgenic rice produced will be grown in the Phytotron, a facility with
containment capabilities.

2. The flowers of the plants will be bagged to prevent spread of pollen in the environment.

3. Seeds will be harvested, stored and analyzed for successful transformation and enhanced resistance to insects and diseases.
4. All other materials will be destroyed by incineration.
5. Incineration of all plant tissues and autoclaving of soil and water used in the experiment.
Conditions for Approval N/A
Date Received 1993-06-11
Date of Approval 1993-07-28

 


Regulated Materials


Regulated Materials Plasmids pCIB1322, pCIB4418, pMU388, p35S- mtlD, lambda EMBL3 library, lambda EMBL3- T7/ Sp6 library
Source N/A
Subject Request of Dr. John Bennett to: a) import plasmids pCIB1322, pCIB4418, pMU388, p35S- mtlD, lambda EMBL3 library, lambda EMBL3- T7/ Sp6 library; b) use the following plasmids in biolistic transformation of rice together with 3 other plasmids which will be
Requesting Party Dr. John Bennett, Senior Molecular Biologist, IRRI
Action of NCBP Approved for implementation

For issuance of permit to import Plasmids pCIB1322, pCIB4418, pMU388, p35S- mtlD, lambda EMBL3 library, lambda EMBL3- T7/ Sp6 library and Transgenic rice seeds by the Plant Quarantine Services, Bureau of Plant Industry.