Project Information

Reference Number 20050198
Project Title Effect of endophyte Azoarcus sp. on gene expression in rice
Project Type Contained Use (Laboratory)
Status Completed
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution Dr. Barbara Reinhold-Hurek University, Bremen, Germany
Supervising IBC IRRI-IBC
Project Leader(s) IRRI, College, Laguna, Philippines
Experimental Facility/Site IRRI, College, Laguna, Philippines
Purpose / Objectives 1. To import the genetically engineered bacterium strain (BHNG3.1) which contains gus marker gene from University of Bremen, Germany. BPI would be requested for the import permit after approval of the proposal by NCBP.

2. To monitor the response of rice (O. sativa) roots towards the colonization by plant growth enhancing nitrogen-fixing endophytes, which colonize rice roots in high numbers without being a pathogen.

3. To compare the response of Azoarcus measured in terms of expression of the gus gene in a microarray analysis with defense responses caused by jasmonic acid and by the pathogen Xanthomonas oryzae.
Biosafety measures Authorized personnels who are experienced in the handling of microorganisms and molecular biological materials (B. Reinhold-Hurek and C. Vera Cruz) will grow Azoarcus and Xanthomonas in the Molecular Pathology Laboratory (EPPD, IRRI)

Inoculated seedlings will be maintained throughout the experiments in sterile test tubes and kept in growth cabinets in phytotron, and only the authorized personnel will be involved in the experiments

All biological waste will be placed in plastic bags and autoclaved.

Glassware will be autoclaved after each use.

Upon completion of the experiments within 12 weeks, all biological material will be destroyed by autoclaving.

All safety measures in the conduct of the experiment and the disposal of waste as contained previous application will be followed.
Conditions for Approval N/A
Date Received 2005-08-01
Date of Approval 2005-08-05


Regulated Materials

Regulated Materials g netically engineered bacterium strain (BHNG3.1) which contains gus marker gene
Source Dr. Barbara Reinhold-Hurek, University of Bremen, Germany
Subject Request of Dr. Darshan Brar for NCBP review and approva
Requesting Party Dr. Darshan S. Brar, Chairperson, IRRI-IBC
Action of NCBP Approved for implementation.

For issuance of permit to import genetically engineered bacterium strain (BHNG3.1) which contains gus marker gene by the Plant Quarantine Service, Bureau of Plant Industry (BPI)