|Project Title||Cloning and expression of a thermophilic lipase gene from Geobacillus sp., M5 and characterization of the recombinant enzyme|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Banos, College, Laguna,049-536-2721; 536-2724|
|Project Leader(s)||Jessica F. Simbahan, University Researcher II|
|Experimental Facility/Site||BIOTECH-UPLB, Laguna, Philippines|
|Purpose / Objectives||a. To clone and express the gene for thermophilic lipase from Geobacillus sp. M5 in E.coli.
b. To purify cloned and wild type thermophilic lipase from M5.
c. To characterize crude extract and purified native lipase from Geobacillussp. M5.
d. To characterize purified recombinant lipase and compare it to the native thermophilic enzyme.
|Conditions for Approval||N/A|
|Date of Approval||2005-11-26|
|Subject||Request for a permit from NCBP to conduct the studies outlined in the proposal|
|Requesting Party||Dr. Jessica F. Simbahan, University Researcher II, BIOTECH-UPLB|
|Action of NCBP||Approved for implementation|