Project Information

Reference Number 20050200
Project Title Cloning and expression of a thermophilic lipase gene from Geobacillus sp., M5 and characterization of the recombinant enzyme
Project Type Contained Use (Laboratory)
Status Terminated
Name of Institution National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Banos, College, Laguna,049-536-2721; 536-2724
Cooperating Institution none
Supervising IBC UPLB-IBC
Project Leader(s) Jessica F. Simbahan, University Researcher II
Experimental Facility/Site BIOTECH-UPLB, Laguna, Philippines
Purpose / Objectives a. To clone and express the gene for thermophilic lipase from Geobacillus sp. M5 in E.coli.
b. To purify cloned and wild type thermophilic lipase from M5.
c. To characterize crude extract and purified native lipase from Geobacillussp. M5.
d. To characterize purified recombinant lipase and compare it to the native thermophilic enzyme.
Biosafety measures N/A
Conditions for Approval N/A
Date Received 2005-11-17
Date of Approval 2005-11-26


Regulated Materials

Regulated Materials N/A
Source N/A
Subject Request for a permit from NCBP to conduct the studies outlined in the proposal
Requesting Party Dr. Jessica F. Simbahan, University Researcher II, BIOTECH-UPLB
Action of NCBP Approved for implementation