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Project Information


Reference Number 20060211
Project Title Identification and Characterization of Rice Promoters
Project Type Contained Use
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution Dr. Alex Johnson
Supervising IBC Biosafety of Committee of IRRI
Project Leader(s) Philip Herve, Molecular Biologist
Experimental Facility/Site • Experiments will be done in the Laboratory of Biotechnology, PBGB Division, IRRI
Purpose / Objectives 1. To identify new rice regulatory elements from publicly available GAL4-GFP rice collection.
2. To characterize the activity of new regulatory elements in rice
Biosafety measures 1. Primary transformants will be evaluated for DNA integration. The PCR and southern experiments will be done in the Biotechnology laboratory.
2. Selected transformants will be evaluated for regulatory element activity. These experiments will be conducted in the approved transgenic facilities (Greenhouse CL4 and screnhouses CS01/CS07)
3. All vectors will be kept in the plasmid collection of the Biotechnology laboratory.
4. All transgenic seeds will be stored in the central transgenic seeds storage facility with restricted access.
5. All transformants (plant tissues) except T1 seeds and subsequent progenies will be autoclaved after analysis. Soil used to grow transgenic plants will be autoclaved prior and after use.
Conditions for Approval N/A
Date Received 2006-00-11
Date of Approval 2006-00-19

 


Regulated Materials


Regulated Materials Transgenic Nipponbare seeds containing the GAL4-GFP
Source Australian Centre For Plant Functional Genomics, Adelaide Australia
Subject Request for review and approval of the NCBP
Requesting Party Dr. Darshan Brar, Chairman, IRRI- IBC
Action of NCBP Approved for implementation subject to compliance with the biosafety measures indicated in the proposal

For issuance of permit to import by the Plant Quarantine Services, Bureau of Plant Industry, the above regulated materials to be used in the research.