Project Information

Reference Number 20060213
Project Title Use of RNA Interference to Characterize Genes Relevant to Achieving Synthetic Aproximix for Hybrid Rice
Project Type Contained Use
Status N/A
Name of Institution International Rice Research Institute, CIMMYT, Mexico
Cooperating Institution CSIRO Plant Industry, Canberra, Australia (Dr. Abdul Chaudhury and Dr. Dr. M
Supervising IBC Biosafety Committee of IRRI
Project Leader(s) John Bennet, Senior Molecular Biologist
Experimental Facility/Site Plant Molecular Biology Laboratory (PMBL), Plant Breeding, Genetics and Biotechnology (PBGB) Division at IRRI
Purpose / Objectives 1. To use the pANDA vector to generate ~50 RNA interference (RNAi) lines for each selected cell cycle-related gene
2. To characterize the lines for alterations in cell cycle and reproduction in anthers and ovule.
Biosafety measures 1. Transgenic plants (generation T0-T2) will be kept in the CL4 transgenic greenhouse, where a security system prevents access of unauthorized personnel.
2. The plant materials, pots and soil for disposal from CL4 will be placed in sealed plastic bags and autoclaved at high temperature and high pressure.
3. Transgenic seeds harvested from CL4 will be kept in hermetically sealed aluminum foil packages and stored in locked refrigerators designated for storage of transgenic seeds in TCGE Laboratory of IRRI. Movement of all materials will be done in compliance of all relevant biosafety and phytosanitary requirements of the Philippines.
Conditions for Approval N/A
Date Received 2006-12-11
Date of Approval 2006-12-19


Regulated Materials

Regulated Materials N/A
Source N/A
Subject Request for review and approval of the NCBP
Requesting Party Dr. Darshan S. Brar, Chairperson, IRRI-IBC
Action of NCBP Approved for implementation subject to compliance with the biosafety measures indicated in the proposal.