Project Information

Reference Number 20070222
Project Title Production of transgenic rice using artificial microRNAs and validation of gene silencing
Project Type Contained Use
Status Completed
Name of Institution International Rice Research Institute (IRRI), Los Banos, Laguna
Cooperating Institution Max Plant Institute (MPI)
Supervising IBC IRRI Biosafety Committee
Project Leader(s) Philippe Herve, Molecular Biologist, IRRI
Experimental Facility/Site Biotechnology Laboratory, PBGB Division, IRRI, transgenic greenhouses (CS01/CS07), transgenic rice seeds will be stored in the centralized transgenic seeds storage room
Purpose / Objectives *To make new vectors containing amiRNA Cassettes
*To generate transgenic rice plants expressing the amiRNAs
*To evaluate the endogenous gene expression of the target genes
*To make available to the rice research community a new gene silencing technology
Biosafety measures *Primary transformants will be evaluated for DNA integration. The PCR and southern experiments will be done in the Biotechnology laboratory.
*Selected transformants will be evaluated for gene expression of the target genes. These experiments will be conducted in the approved transgenic facilities (Greenhouses C14 and screenhouses CS01/CS07)
*All vectors will be kept in the plasmid collection of the Biotechnology laboratory.
*All transgenic seeds will be stored in the central transgenic seeds storage facility with restricted access.
*All transformants (plant tissues) except T1 seeds and subsequent progenies will be autoclaved after analysis. Soil used to grow transgenic plants will also be autoclaved prior and after use.
Conditions for Approval none
Date Received 2007-09-21
Date of Approval 2007-10-13


Regulated Materials

Regulated Materials N/A
Source N/A
Subject For NCBP approval of the revised proposal
Requesting Party Dr. Darshan S. Brar, Chairperson, IRRI-IBC
Action of NCBP Approved for implementation subject to compliance with all biosafety measures indicated in the proposal.