|Project Title||Evaluation of Transgenic Rice Plants with Synthetic Version of Cry1A(b) Gene from Bacillus thuringiensis for Resistance to Yellow Stem Borer (Scirpophaga incertulas), Striped Stem Borer (Chilo suppressalis), Rice Leaffolders (Cnaphalocrocis medinalis and|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Cooperating Institution||Plantech Research Institute Yokohama 227, Japan|
|Supervising IBC||IRRI- IBC|
|Project Leader(s)||John Bennett, Senior Molecular Biologist|
|Experimental Facility/Site||Philippines (IRRI and NCBL)|
|Purpose / Objectives||To evaluate transgenic rice for resistance to insect pests.|
|Biosafety measures||1. Plants will be kept in mylar containers in the Tissue Culture Laboratory of the NCBL Annex during testing for resistance to the insects. Plants will be placed in a screen cage during growth to seed in the IRRI Phytotron.
2. At the flowering stage, every panicle will be bagged to prevent cross pollination or escape of pollen.
3. Plants and insects used in the experiments will be destroyed by incineration after use.
4. The seeds will be kept in sealed plastic bags inside a sealed metal container and stored in a refrigerator in the Plant Molecular Biology Laboratory.
|Conditions for Approval||Should comply with the guidelines endorsed by NCBP|
|Date of Approval||1993-10-29|
|Regulated Materials||100 seeds of transgenic rice Oryza sativa|
|Source||Plantech Research Institute, 1000 Kamoshida, Midori- ku, Yokohama, Japan|
|Subject||Dr. John Bennett, to import seeds of transgenic rice expressing an insecticidal protein gene, a synthetic version of the Gene from Bacillus thuringiensis|
|Requesting Party||Dr. John Bennett, Senior Molecular Biologist, IRRI|
|Action of NCBP||Approved for implementation
For issuance of permit to import 100 seeds of transgenic rice by the Plant Quarantine Services, Bureau of Plant Industry.