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Project Information


Reference Number 20080227
Project Title Development of Regeneration System for Biolistic-mediated Transformation for Abaca (Musa textiles Nee) Resistant to Abaca Bunchy Top Virus (ABTV)
Project Type Contained Use
Status completed
Name of Institution Crop Science Cluster
Cooperating Institution 1424 Quezon Ave., Quezon City
Asia Trust Bank Annex Building
Fiber Industry Development Authority (FIDA), Quezon City
National Institute of Molecular Biology and Biotechnology (NIMBB), UP Diliman, Quezon City
• DR. VERMANDO M. AQUINO
• MS. JOSEPHINE B. REGALADO
Supervising IBC UPLB-IBC
Project Leader(s) Evalour T. Aspuria, Ph.D
Experimental Facility/Site • Plant Tissue Culture Laboratory
Purpose / Objectives • To develop the regeneration system for biolistic-mediated transformation of abaca resistant to abaca bunchy top virus.
• To induce scalp and globule formation for the establishment of embryogenic cell suspension cultures for transformation.
• To induce somatic embryogenesis in bombarded embryogenic cell cultures.
• To germinate, select and micropropagate putative abaca transformants resistant to abaca bunchy top virus (ABTV).
Biosafety measures • Containment capabilities of laboratory
The putative transgenic plants will be contained in the IPB-CSC Biochemistry Laboratory.
• Sterilization procedures
All experimental plants, including media, and glassware will be sterilized or autoclaved.
• Personnel awareness of biosafety procedures
All personnel involved are aware of the biosafety procedures.
• Past history of biosafety in laboratory
The IPB-CSC Biochemistry Laboratory is already certified by NCBP for use in genetic engineering studies and had a good history of biosafety such as all wastes are decontaminated, laboratory gowns are available, insects and rodents control.
• Labeling/designation of risk areas
To be followed
• Decontamination facilities
Autoclaves will be provided in the IPB-CSC Biochemistry Laboratory.
• “Biosafetiness” of equipment
The IPB-CSC Biochemistry Laboratory equipment follows standard specifications.
Conditions for Approval N/A
Date Received 2008-01-24
Date of Approval 2008-05-24

 


Regulated Materials


Regulated Materials N/A
Source N/A
Subject UPLB-IBC’s endorsement of the proposal for NCBP approval.
Requesting Party Dr. Adelina A. Barrion, Chairman, UPLB-IBC
Action of NCBP Approved for implementation