Project Information

Reference Number 19930020
Project Title Application and Improvement of Biolistic Transformation Methods (Addendum II)
Project Type Contained Use (Laboratory)
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution none
Supervising IBC IRRI- IBC
Project Leader(s) John Bennett, Senior Molecular Biologist
Experimental Facility/Site Philippines (IRRI)
Purpose / Objectives 1. Development of an efficient system for genetic engineering of rice using the bacterial bar gene as a selectable marker gene during the initial stage of transformation.
2. Development of a protocol for removing the bar gene from transgenic plants by conventional segregation.
Biosafety measures 1. The transgenic rice produced will be grown in the Phytotron, a facility with
containment capabilities.

2. The flowers of the plants will be bagged to prevent spread of pollen in the environment.

3. Seeds will be harvested, stored and analyzed for successful transformation and enhanced resistance to insects and diseases.

4. All other materials will be destroyed by incineration.
Conditions for Approval N/A
Date Received 1993-08-30
Date of Approval 1993-10-29


Regulated Materials

Regulated Materials 100 ug of pUBA, pDMC205, pDMC203, pDMC204, p35S GUS INT, and pDM302
Source Center of Application, Molecular Biology to International Agriculture, PO Box 3200 Canberra, ACT 2601 Australia
Subject Clearance to use the plasmids brought in by Dr. Richard A. Jefferson, Director of CAMBIA for the research entitled “Application and Improvement of Biolistic Transformation Methods (Addendum 1)
Requesting Party Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI
Action of NCBP Approved for implementation

For issuance of permit to import 100 ug of pUBA, pDMC205, pDMC203, pDMC204, p35S GUS INT, and pDM302 by the Plant Quarantine Services, Bureau of Plant Industry