|Project Title||Application and Improvement of Biolistic Transformation Methods (Addendum II)|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Supervising IBC||IRRI- IBC|
|Project Leader(s)||John Bennett, Senior Molecular Biologist|
|Experimental Facility/Site||Philippines (IRRI)|
|Purpose / Objectives||1. Development of an efficient system for genetic engineering of rice using the bacterial bar gene as a selectable marker gene during the initial stage of transformation.
2. Development of a protocol for removing the bar gene from transgenic plants by conventional segregation.
|Biosafety measures||1. The transgenic rice produced will be grown in the Phytotron, a facility with
2. The flowers of the plants will be bagged to prevent spread of pollen in the environment.
3. Seeds will be harvested, stored and analyzed for successful transformation and enhanced resistance to insects and diseases.
4. All other materials will be destroyed by incineration.
|Conditions for Approval||N/A|
|Date of Approval||1993-10-29|
|Regulated Materials||100 ug of pUBA, pDMC205, pDMC203, pDMC204, p35S GUS INT, and pDM302|
|Source||Center of Application, Molecular Biology to International Agriculture, PO Box 3200 Canberra, ACT 2601 Australia|
|Subject||Clearance to use the plasmids brought in by Dr. Richard A. Jefferson, Director of CAMBIA for the research entitled “Application and Improvement of Biolistic Transformation Methods (Addendum 1)|
|Requesting Party||Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI|
|Action of NCBP||Approved for implementation
For issuance of permit to import 100 ug of pUBA, pDMC205, pDMC203, pDMC204, p35S GUS INT, and pDM302 by the Plant Quarantine Services, Bureau of Plant Industry