|Project Title||Genetic Analysis of Philippine Strains of Pseudomonas solanacearum: Banana (bugtok) Strains|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||Institute of Biological Sciences (IBS),UP Los Banos, College, Laguna,(49) 536-3438|
|Cooperating Institution||Australian Center for International Agricultural Research (ACIAR) National Institute of Biotechnology and Applied Microbiology (BIOTECH)|
|Supervising IBC||UPLB- IBC, IRRI- IBC|
|Project Leader(s)||Asuncion K. Raymundo, Associate Professor,IBS-UPLB|
|Experimental Facility/Site||(BIOTECH and IBS- UPLB) Philippines|
|Purpose / Objectives||1. To analyze genetic variation in Pseudomonas solanacearum
2. To evaluate the population structure of Philippine Bugtok strains in comparison to other strains/ Races/ biovars of the organisms.
|Biosafety measures||1. Laboratory personnel will be oriented on proper procedure in sterilization and Decontamination and in the containment of organisms to be used.
2. DNA manipulation experiments will be done at BIOTECH and at IBS Research Laboratory equipped with isolation chambers and incubators for culture, transfer, and storage.
3. Pathogenicity testing will be conducted in greenhouse. Used plastic containers and pots will be decontaminated by using 20% sodium hypochlorite. Plants will be burned and soil will be sterilized by dry heating for several hours.
|Conditions for Approval||N/A|
|Date of Approval||1994-07-01|
|Subject||Letter from Dr. William G. Fernandez, Chairman of the UPLB- IBC, endorsing the research project of Dr. Asuncion K. Raymundo, Associate Professor, IBS- UPLB for NCBP clearance.|
|Requesting Party||Dr. Asuncion K. Raymundo, Associate Professor, IBS- UPLB|
|Action of NCBP||Approved for implementation|