Project Information

Reference Number 19940030
Project Title 1. Production of Novel Male-Sterile Transgenic Rice with rol-b Gene (under the Cross-Ecosystems Program) 2) Production of Dwarf Aromatic Transgenic Rice (under the Irrigated Rice Ecosystem Program
Project Type Contained Use (Laboratory)
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution 1. Institute of Biological Chemistry 2. Institute of Molecular and Cellular Biosciences Washington State University
Supervising IBC IRRI-IBC
Project Leader(s) John Bennett, Senior Molecular Biologist
Experimental Facility/Site IRRI, Philippines
Purpose / Objectives 1.To produce novel male- sterile transgenic rice engineered with the rol B gene of Agrobacterium rhizogenes and evaluate stability of male- sterility.

2.To produce dwarf aromatic transgenic rice engineered with the rol C gene of Agrobacterium rhizogenes and evaluate performance for enhanced yield potential.
Biosafety measures 1. The tested plants will be kept in isolation in the Phytotron or CL4 Greenhouse to prevent escape of pollen and access by unauthorized personnel.
2. Panicles of flowering plants will be bagged as an additional precaution against escape of pollen.
3. The plant materials for disposal will be placed in sealed plastic bags and autoclaved prior to incineration.
4. Pots and soil will be autoclaved at high temperature and high pressure after use.
5. Transgenic seeds will be kept in hermetically sealed aluminum foil package, inside a sealed metal container and stored in locked refrigerators designated for storage of transgenic seeds in the Tissue Culture Laboratory and the Plant Molecular Biology Laboratory.
Conditions for Approval N/A
Date Received 1994-07-24
Date of Approval 1994-10-26


Regulated Materials

Regulated Materials rol B gene pGA1322/ pGA 1319 and rol C gene PTMI
Source Washington State University, USA and University of Tokyo, Japan
Subject Request of Dr. John Bennett to import rol B (indole- acetic acid- B- glucosidase) and rol C gene (cytokinin- B- glucosidase PTMI)
Requesting Party Dr. John Bennett, Senior Molecular Biologist, IRRI
Action of NCBP Approved for implementation

For issuance of permit to import rol B gene pGA1322/ pGA 1319 and rol C gene PTMI by the Plant Quarantine Services, Bureau of Plant Industry