|Project Title||Assessing Opportunities for Biological Nitrogen Fixation in Rice|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Cooperating Institution||CNRS- INRA, France; MPI, Germany|
|Project Leader(s)||J. K. Ladha, Soil Microbiologist|
|Experimental Facility/Site||IRRI, Philippines|
|Purpose / Objectives||1. To request NCBP recommendation for issuance of permit to import diazotrophic microorganisms and Enod::gus constructs and allow the conduct of experiments using said strains as well as transform the rice with Enod::gus gene constructs for experimental purposes.
2. Proposal 3: Request for Import of E. coli Strains, Harbouring Nodulation/ Marker Gene Constructs to Be used for Biolistic Transformation of Rice.
3.To establish naturally occurring NH4+ excreting diazotrophs endophytically in rice roots.
4.To identify nitrogen- fixing bacteria which can form nodules or symbiotic association with rice.
|Biosafety measures||a) that the Agrobacterium nitrobacter (if pathogenic) will not be part of the wild type nitrogen fixing microorganisms to be imported.
b) IRRI shall provide a description of the containment facilities in the laboratory where the experiments will be conducted; and
c) autoclaving and incineration of all plant tissues and soil used in the experiment shall be supervised by the Plant Quarantine Service.
|Conditions for Approval||N/A|
|Date of Approval||1994-08-22|
|Regulated Materials||N-2 fixing wild type, non- pathogenic microorganisms, genetically modified non- pathogenic N-2 fixing organisms; and E. coli strains harboring marker gene constructs|
|Source||Agricultural University, Netherlands|
|Subject||Request of Dr. F.A. Bernardo to import N-2 fixing wild type, non- pathogenic microorganisms; genetically modified non- pathogenic N-2 fixing organisms; and E. coli strains harboring marker gene constructs.|
|Requesting Party||Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI|
|Action of NCBP||Approved for implementation
For issuance of permit to import N-2 fixing wild type, non- pathogenic microorganisms; genetically modified non-pathogenic N-2 fixing organisms; and E. coli strains harboring marker gene constructs by the Plant Quarantine Services, Bureau of Plant Industry