Project Information
| Reference Number | 19940031 |
| Project Title | Assessing Opportunities for Biological Nitrogen Fixation in Rice |
| Project Type | Contained Use (Laboratory) |
| Status | N/A |
| Name of Institution | International Rice Research Institute (IRRI), Los Baños, Laguna |
| Cooperating Institution | CNRS- INRA, France; MPI, Germany |
| Supervising IBC | IRRI-IBC |
| Project Leader(s) | J. K. Ladha, Soil Microbiologist |
| Experimental Facility/Site | IRRI, Philippines |
| Purpose / Objectives | 1. To request NCBP recommendation for issuance of permit to import diazotrophic microorganisms and Enod::gus constructs and allow the conduct of experiments using said strains as well as transform the rice with Enod::gus gene constructs for experimental purposes. 2. Proposal 3: Request for Import of E. coli Strains, Harbouring Nodulation/ Marker Gene Constructs to Be used for Biolistic Transformation of Rice. 3.To establish naturally occurring NH4+ excreting diazotrophs endophytically in rice roots. 4.To identify nitrogen- fixing bacteria which can form nodules or symbiotic association with rice. |
| Biosafety measures | a) that the Agrobacterium nitrobacter (if pathogenic) will not be part of the wild type nitrogen fixing microorganisms to be imported. b) IRRI shall provide a description of the containment facilities in the laboratory where the experiments will be conducted; and c) autoclaving and incineration of all plant tissues and soil used in the experiment shall be supervised by the Plant Quarantine Service. |
| Conditions for Approval | N/A |
| Date Received | 1994-08-04 |
| Date of Approval | 1994-08-22 |
Regulated Materials
| Regulated Materials | N-2 fixing wild type, non- pathogenic microorganisms, genetically modified non- pathogenic N-2 fixing organisms; and E. coli strains harboring marker gene constructs |
| Source | Agricultural University, Netherlands |
| Subject | Request of Dr. F.A. Bernardo to import N-2 fixing wild type, non- pathogenic microorganisms; genetically modified non- pathogenic N-2 fixing organisms; and E. coli strains harboring marker gene constructs. |
| Requesting Party | Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI |
| Action of NCBP | Approved for implementation For issuance of permit to import N-2 fixing wild type, non- pathogenic microorganisms; genetically modified non-pathogenic N-2 fixing organisms; and E. coli strains harboring marker gene constructs by the Plant Quarantine Services, Bureau of Plant Industry |