|Project Title||CE91- 2-1/ Genome Manipulation Activity #8. Application and Improvement of Transformation Methods|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Supervising IBC||IRRI,College, Laguna|
|Project Leader(s)||Francisco J. Zapata, Tissue Culture Specialist, IRRI|
|Experimental Facility/Site||IRRI, Philippines|
|Purpose / Objectives||1. To develop a saturated genetic map of rice and use it to locate genes governing traits of agronomic importance.
2. To supplement the sources of genetic variation available to rice researchers and farmers.
3.Optimization of conditions for PEG- mediated and electroporation transformation using plasmid DNA containing the rice amylase promoter/ GUS fusion gene.
4. Regeneration of transformed calli of selected japonica and indica varieties.
|Biosafety measures||1. Plants transformed with the plasmid will be grown in a containment facility.
2. Panicles of flowering plant will be bagged to prevent release of pollen.
3. Plants used in transformation studies will be destroyed after experiment.
|Conditions for Approval||Should comply with the guidelines endorsed by NCBP|
|Date of Approval||1992-02-18|
|Regulated Materials||10 ug of Plasmid DNA containing the Rice amylase promoter/ GUS fusion gene.|
|Source||University of California, Davis, USA|
|Subject||Request to import plasmid DNA containing the rice amylase promoter/ GUS fusion gene|
|Requesting Party||Dr. Francisco J. Zapata, Tissue Culture Specialist,IRRI|
|Action of NCBP||Approved for implementation|