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Project Information


Reference Number 19940036
Project Title cDNA Library from Midgut of Rice Yellow Stemborer/ CE- 2: Biotechnology Tools for Rice Breeding
Project Type Contained Use (Laboratory)
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution none
Supervising IBC IRRI-IBC
Project Leader(s) John Bennett, Senior Molecular Biologist
Experimental Facility/Site Philippines (IRRI)
Purpose / Objectives Preparation of cDNA Library from Midgut of Rice Yellow Stem Borer
Biosafety measures 1. The cloning will be conducted in the Plant Molecular Biology Lab. (PMBL) at IRRI, a P2 facility.
2. The cDNA library will be stored in a locked freezer at PMB Lab.
3. The rDNA manipulation will be carried out in the Biosafety hood of PMBL.
4. At the recommendation of IRRI Biosafety Committee, the library will be kept in the –80ºC freezer of the Plant Molecular Biology Lab. and will be available to other scientists to conduct research upon approval of the NCBP.
5. Selected clones will be extracted with phenol for phagemid preparation. This will kill the clones.
6. All plant tissues will be incinerated
and soil and water used in the
experiment will be autoclaved.
Conditions for Approval N/A
Date Received 1994-11-27
Date of Approval 1995-02-20

 


Regulated Materials


Regulated Materials one vial each of: a) E coli strain NM522; b) Phagemid directional cloning vector; c) Directional cloning toolbox
Source Pharmacia Biotech, Hongkong
Subject Request of Dr. F.A. Bernardo to import one vial each of: a) E coli strain NM522; b) Phagemid directional cloning vector; c) Directional cloning toolbox
Requesting Party Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI
Action of NCBP Approved for implementation

For issuance of permit to import one vial each of: a) E coli strain NM522
b) Phagemid directional cloning vector
c) Directional cloning toolbox by the Plant Quarantine Services, Bureau of Plant Industry