|Project Title||Production of Transgenic Rice Using Agrobacterium Mediated Transforming Systems|
|Project Type||Contained Use (Laboratory)|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Project Leader(s)||Swapan K. Datta, Plant Biotechnologist|
|Experimental Facility/Site||IRRI, Philippines|
|Purpose / Objectives||To produce transgenic rice engineered using Agrobacterium transformation methods.|
|Biosafety measures||1. The tested plants will be kept in isolation in the Phytotron or transgenic greenhouse to prevent escape of pollen and access by unauthorized personnel.
2. Panicles of flowering plants will be bagged as an additional precaution against escape of pollen.
3. Plant materials for disposal will be placed in sealed plastic bags and autoclaved prior to incineration.
4. Pots and soil will be autoclaved at high temperature and high pressure after use.
5. Transgenic seeds will be kept in hermetically sealed aluminum foil packages, inside a sealed metal container and stored in locked refrigerators/ chambers designated for storage of transgenic seeds in the Tissue Culture Laboratory of IRRI.
|Conditions for Approval||N/A|
|Date of Approval||1995-11-09|
|Regulated Materials||10- 100 ug Agrobacterium tumefacienns LBA440 (pTOK233)|
|Source||Japan Tobacco, Inc.|
|Subject||Request of Dr. F.A. Bernardo to import plasmid DNA’s (10- 100 ug in microtubes)|
|Requesting Party||Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI|
|Action of NCBP||Approved for Implementation
For issuance of permit to import 10- 100 ug Agrobacterium tumefacienns LBA440 (pTOK233) by the Plant Quarantine Services, Bureau of Plant Industry