|Project Title||Independent Inoculation of Rice Tungro Viruses Using Agrobacterium- Mediated Transfer Systems|
|Project Type||Contained Use|
|Name of Institution||International Rice Research Institute (IRRI), Los Baños, Laguna|
|Cooperating Institution||1. John Innes Institute, Norwich, England
2. University of Wisconsin, Madison
|Project Leader(s)||Ossmat Azzam, Tissue Culture Specialist|
|Experimental Facility/Site||IRRI, Philippines|
|Purpose / Objectives||1. To inoculate rice plants with rice tungro viruses using Agrobacterium without the need of green leafhoppers.
2. To screen improved breeding lines for their resistance ability to rice tungro viruses independently from the vector.
|Biosafety measures||1. The inoculated plants will be kept in CL4 facility and only authorized personnel will handle the experiments.
2. Plants will not be kept to beyond six weeks post inoculation.
3. At the end of the experiments, plant materials will be placed in plastic bags, autoclaved prior to be discarded.
4. Pots and soil will be autoclaved at
high temperature and pressure after
|Conditions for Approval||NCBP will be informed on the initial experiments with the disarmed plasmids as well as the time when the proponent stops using the C58 for inoculation. The said information should be included in IRRI- IBC’s regular report to the NCBP.|
|Date of Approval||1996-11-19|
|Regulated Materials||10- 100 ug/ microtube Agrobacterium tumefaciens C- 58 (pTIC58) pGV3850, pBIN19|
|Source||University of Wisconsin- Madison; Norwich, United Kingdom|
|Subject||Request of Dr. Ossmat Azzam to import 10- 100 ug/ microtube Agrobacterium tumefaciens C- 58 (pTIC58) pGV3850, pBIN19|
|Requesting Party||Dr. Ossmat Azzam, Tissue Culture Specialist,IRRI|
|Action of NCBP||Approved for Implementation
For issuance of permit to import 10- 100 ug/ microtube Agrobacterium tumefaciens C- 58 (pTIC58) pGV3850, pBIN19 by the Plant Quarantine Services, Bureau of Plant Industry