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Project Information


Reference Number 19960065
Project Title Independent Inoculation of Rice Tungro Viruses Using Agrobacterium- Mediated Transfer Systems
Project Type Contained Use
Status N/A
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution 1. John Innes Institute, Norwich, England
2. University of Wisconsin, Madison
Supervising IBC IRRI-IBC
Project Leader(s) Ossmat Azzam, Tissue Culture Specialist
Experimental Facility/Site IRRI, Philippines
Purpose / Objectives 1. To inoculate rice plants with rice tungro viruses using Agrobacterium without the need of green leafhoppers.
2. To screen improved breeding lines for their resistance ability to rice tungro viruses independently from the vector.
Biosafety measures 1. The inoculated plants will be kept in CL4 facility and only authorized personnel will handle the experiments.
2. Plants will not be kept to beyond six weeks post inoculation.
3. At the end of the experiments, plant materials will be placed in plastic bags, autoclaved prior to be discarded.
4. Pots and soil will be autoclaved at
high temperature and pressure after
each use.
Conditions for Approval NCBP will be informed on the initial experiments with the disarmed plasmids as well as the time when the proponent stops using the C58 for inoculation. The said information should be included in IRRI- IBC’s regular report to the NCBP.
Date Received 1996-09-20
Date of Approval 1996-11-19

 


Regulated Materials


Regulated Materials 10- 100 ug/ microtube Agrobacterium tumefaciens C- 58 (pTIC58) pGV3850, pBIN19
Source University of Wisconsin- Madison; Norwich, United Kingdom
Subject Request of Dr. Ossmat Azzam to import 10- 100 ug/ microtube Agrobacterium tumefaciens C- 58 (pTIC58) pGV3850, pBIN19
Requesting Party Dr. Ossmat Azzam, Tissue Culture Specialist,IRRI
Action of NCBP Approved for Implementation

For issuance of permit to import 10- 100 ug/ microtube Agrobacterium tumefaciens C- 58 (pTIC58) pGV3850, pBIN19 by the Plant Quarantine Services, Bureau of Plant Industry