Project Information

Reference Number 19960069
Project Title Molecular Cloning of Amylase Gene(s) from Bacillus subtilis and its Expression in Escherichia coli
Project Type Contained Use
Status N/A
Name of Institution National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Banos, College, Laguna,049-536-2721; 536-2724
Cooperating Institution N/A
Supervising IBC UPLB-IBC
Project Leader(s) Reynaldo V. Ebora, University Researcher
Experimental Facility/Site BIOTECH-UPLB, Laguna
Purpose / Objectives 1. To purify and characterize amylase enzyme from local Bacillus subtilis.
2. To clone and characterize the amylase gene from Bacillus subtilis.
3. To express the amylase gene (s) in E. coli and characterize the gene product.
4. To determine the fermentation conditions optimum for amylase production of the genetically- engineered E. coli.
Biosafety measures 1. Containment capabilities
2. Sterilization Procedures
3. Personnel awareness of biosafety procedures
4. Labeling/ designation of “risk” areas
5. Decontamination facilities
6. “Biosafetiness” of equipment
Conditions for Approval N/A
Date Received 1996-11-05
Date of Approval 1996-12-12


Regulated Materials

Regulated Materials SURE cells
Source Stratagene Cloning Systems, North Toney Pine Road, La Jolla, California
Subject Request of Dr. Ida Dalmacio to import SURE cells
Requesting Party Dr. Ida Dalmacio, Chairperson, UPLB-IBC
Action of NCBP Approved for Implementation

For issuance of permit to import SURE cells by the Plant Quarantine Services, Bureau of Plant Industry (BPI)