Project Information

Reference Number 19960071
Project Title Transformation of E. coli JM109 with Plasmid Borne Bioluminiscent Gene (previously referred to as UNILAB Mobile Biotechnology Demonstration Laboratory)
Project Type Contained Use
Status N/A
Name of Institution United Laboratories, Inc. (UNILAB)
Cooperating Institution Center for Biomolecular Science Foundation, Inc. (CBMS)
Supervising IBC Center for Biomolecular Science Foundation, Inc. (CBMS)
Project Leader(s) Lourdes J. Cruz, Professor
Experimental Facility/Site UNILAB, Philippines
Purpose / Objectives 1. To introduce concepts of biotechnology to secondary schools via a series of games , poster exhibits, flyers, and demonstration experiments.
2. To teach basic tools and concepts of biotechnology through simple, hands- on, time- telescoped experiments.
Biosafety measures 1. Containment capabilities
2. Sterilization Procedures
3. Personnel awareness of biosafety procedures
4. Labeling/ designation of “risk” areas
5. Decontamination facilities
6. “Biosafetiness” of equipment
7. Chloroform will not be used in any procedure/ process- and should be replaced with DiChloro Methane.
8. Polyethylene glycol or Glycerol will be used in place of degraded plasmid cells during demonstrations.
Conditions for Approval N/A
Date Received 1996-08-14
Date of Approval 1996-08-20


Regulated Materials

Regulated Materials E. coli strain, JM 109
Source N/A
Subject Request of Dr. Angelita Reyes for endorsement of the Proposal
Requesting Party Dr. Angelita Reyes, Chairperson, CBMS- IBC
Action of NCBP Approved for Implementation

For issuance of permit to import E. coli strain JM 109 by the Plant Quarantine Services, Bureau of Plant Industry