|Project Title||Use of Cluconidase (gus A) Gene as a Marker to Monitor the Fate of Pseudomonas solanacearum E. F. Smith in the Soil and Tomato (Lycopesicon esculentum Mill)|
|Project Type||Contained Use|
|Name of Institution||National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Banos, College, Laguna,049-536-2721; 536-2724|
|Supervising IBC||UPLB- IBC, IRRI- IBC|
|Project Leader(s)||Rosario Monsalud, University Researcher|
|Experimental Facility/Site||Philippines (BIOTECH- UPLB)|
|Purpose / Objectives||1. To introduce the reporter gene B- glucuronidase from E. coli to P. solanacearum.
2. To monitor and compare the colonization behavior/ pattern of the labeled strain in susceptible and resistant tomato varieties.
3. To determine the effects of temperature and moisture on the population of P. solanacearum in the soil.
|Biosafety measures||1. Containment capabilities
2. Sterilization procedures
3. Personnel awareness of biosafety procedures
4. Labelling/ designation of “risk” areas
5. Decontamination facilities
6. “Biosafetiness” of equipment
|Conditions for Approval||The committee further recommended that the gantt chart be modified to indicate where each activity is going to be conducted for the purpose of determining which among those will be carried out in a contained facility.|
|Date of Approval||1997-10-13|
|Subject||Letter of Dr. Ida Dalmacio for proposal endorsement|
|Requesting Party||Dr. Ida Dalmacio, Chairperson, UPLB-IBC|
|Action of NCBP||Approved for implementation|