|Project Title||Effects of the Snowdrop Lectin on Rice Insect Pests and Their Natural Enemies|
|Project Type||Contained Use|
|Name of Institution||International Rice Research Institute (IRRI) College, Laguna|
|Cooperating Institution||John Innes Centre|
|Supervising IBC||IRRI- IBC|
|Project Leader(s)||Michael Cohen, Entomologist|
|Experimental Facility/Site||IRRI, Philippines|
|Purpose / Objectives||1. To evaluate the effect of rice plants transformed with the snow drop lectin gene on two groups of rice pests and on selected natural enemies that feed on them.
2. To determine if there are negative effects of the toxin on predators and parasitoids that attack rice pests which are feeding on snowdrop lectin- transformed rice.
|Biosafety measures||1. The seeds will be kept in sealed plastic containers in a locked refrigerator in either the Bt lab of the Lampe Laboratory or in the Bt lab in the basement of the NCBrady Building.
2. Plants will be grown only in the CL4 greenhouse or the biosafety room of greenhouse BG08.
3. Plants grown in BG08 will be destroyed before the booting stage.
4. At the flowering stage, every panicle of plants in the CL4 will be bagged to prevent cross- pollination or escape of pollen.
5. Plants used in the experiment will be destroyed by incineration after use.
6. Mobile stages of pest insects and of natural enemies will be kept in cages and destroyed by incineration after use.
|Conditions for Approval||N/A|
|Date of Approval||1998-05-13|
|Regulated Materials||rice seeds: a) transgenic rice seeds from four R1 plants containing the pRSSGNA1 construct and seeds from two R1 plants that do not contain the construct; b) transgenic rice seeds from four R1 plants containing the pUBIGNA1 construct, and seeds from two R|
|Source||John Innes Centre, U.K.|
|Subject||Letter of Dr. F.A. Bernardo for permit to import rice seeds transformed with genes from the snowdrop lectin plant (Galanthus nivalus)|
|Requesting Party||Dr. F.A. Bernardo, Deputy Director General for International Services/ IRRI, IRRI|
|Action of NCBP||Approved for implementation
For issuance of permit to import rice seeds by the Plant Quarantine Services, Bureau of Plant Industry.