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Project Information


Reference Number 19980099
Project Title Investigation of Bacillus thuringiensis- endotoxin Binding to Midgut Receptors of Asiatic Corn Borer Ostrinia furnacalis
Project Type Contained Use (Laboratory)
Status N/A
Name of Institution National Institute of Molecular Biology and Biotechnology (BIOTECH),University of the Philippines Los Banos, College, Laguna,049-536-2721; 536-2724
Cooperating Institution N/A
Supervising IBC UPLB-IBC
Project Leader(s) Edwin Alcantara, University Researcher I
Experimental Facility/Site BIOTECH, Philippines
Purpose / Objectives 1. To identify Cry toxins with high binding affinity to midgut receptors of O. furnacalis.
2. To quantify receptor binding and determine which component of the binding process is critical to a successful Cry toxin- receptor interaction.
3. To develop a molecular approach to improve receptor binding of Cry toxin to O. furnacalis midgut receptors.
4. To determine the role of midgut receptors in the resistance of O. furnacalis to Bt toxin.
Biosafety measures 1. Imported and locally transformed E. coli harboring cloned cry genes will be stored in glycerol at –80ºC freezer.
2. All aseptic work will be done in a laminar flow hood.
3. Growing of recombinant E. coli cells will be done in enclosed shakers with properly regulated speed and temperature.
4. All glasswares and solid and liquid wastes coming from the production of cloned B. thuringiensis endotoxin will be sterilized at 121ºC for 20 minutes in an autoclave.
5. Only researchers with experience in microbiological aspect techniques will be hired in the project.
6. Insect rearing cages with adequate containment will be provided for the rearing of B. thuringiensis toxin resistant insects. A separate rearing room with padlock will be provided for this purpose.
7. Discarded insects will be destroyed by autoclaving in 121ºC for 20 minutes.
Conditions for Approval In the induction of cry toxin resistance in O. furnacalis, cognizant of the importance of conserving the pest population’s susceptible gene pool the NCBP therefore enjoins the UPLB- IBC to closely monitor this resistance selection activity to ensure that the O. furnacalis strains selected for resistance are not allowed to reproduce outside the containment facilities
Date Received 1998-08-26
Date of Approval 1998-09-15

 


Regulated Materials


Regulated Materials cry1Aa, cry1Ab, cry1Ac, cry1B, cry!C, cry1F, cry11A, and cry9 genes cloned into pKK223- 3 and transformed into E. coli J.M. 105
Source Bacillus Genetic Stock Center, Ohio State University
Subject Request of Dr. Agnes Zamora for permit to import cry1Aa, cry1Ab, cry1Ac, cry1B, cry!C, cry1F, cry11A, and cry9 genes cloned into pKK223- 3 and transformed into E. coli J.M. 105
Requesting Party Dr. Agnes Zamora, Chairman, UPLB- IBC
Action of NCBP Approved for Implementation

For issuance of permit to import cry1Aa, cry1Ab, cry1Ac, cry1B, cry1C, cry1F, cry11A, and cry9 genes cloned into pKK223- 3 and transformed into E. coli J.M. 105 explants of transformed bananas by the Plant Quarantine Services, Bureau of Plant Industry.