Project Information

Reference Number 19990110
Project Title Establishment of a Model Biolistics- Based Transformation System of Cultured Plant Cells and Tissues for the Development of Philippine Banana Cultivars through Transgenic Applications
Project Type Contained Use (Laboratory)
Status Completed
Name of Institution National Institute of Molecular Biology and Biotechnology (NIMBB), UP Diliman, Quezon City
Cooperating Institution Department of Science and Technology (DOST),Gen. Santos Ave., Bicutan, Taguig, M. Mla.
Supervising IBC UP Diliman-IBC
Project Leader(s) Surya K. Manohar, Associate Professor
Experimental Facility/Site UP Diliman, Quezon City, Philippines
Purpose / Objectives 1. To establish banana tissue culture facility at NIMBB- UPD.
2. To establish callus cultures from banana/corn tissue.
3. To establish micropropagated banana tissue culture.
4. To establish protocols for obtaining embryogenic cell cultures from banana.
5. To establish protocols for obtaining protoplasts and protoplast cultures.
6. To establish a working protocol for transformation of banana tissue and cells via DNA coated particle delivery using BIORAD PDS- 1000 apparatus.
7. To establish a protocol for regeneration of banana from culture tissue.
8. Evaluating transient and stable transformation in banana tissues by reporter gene assay.
Biosafety measures 1. Containment capability of laboratory
2. Sterilization procedures
3. Personnel awareness of biosafety procedures
4. Labelling/ designation of “risk” areas
5. “Biosafetiness” of equipment
Conditions for Approval The committee has approved the conduct of the experiments, subject to compliance with the provisions in the biosafety guidelines, specifically the required level of containment (BL1) and autoclaving and incineration of plant tissues and soil used in the experiment.
Date Received 1999-05-04
Date of Approval 1999-11-03


Regulated Materials

Regulated Materials N/A
Source N/A
Subject Request of Dr. Elma Llaguno for proposal endorsement
Requesting Party Dr. Elma Llaguno, Chairman, UP Diliman IBC
Action of NCBP Approved for implementation