Project Information

Reference Number 19990111
Project Title Assessing Opportunities for Biological Nitrogen Fixation in Rice- Transformation of Rice with Legume Nodulin Genes to Study the Genetic Predisposition of Rice for Forming Nodular Symbiosis with Rhizobia
Project Type Contained Use (Greenhouse)
Status Completed
Name of Institution International Rice Research Institute (IRRI), Los Baños, Laguna
Cooperating Institution Australian National University, Canberra, Australia;
CSIRO, Canberra, Australia
INRA/CNRS, Toulouse, France;
University of California Davis, USA;
University of Tennessee, Knoxville, USA;
Supervising IBC IRRI-IBC
Project Leader(s) P. M. Reddy, Affiliate Scientist
Experimental Facility/Site IRRI, Philippines
Purpose / Objectives To assess the predisposition of rice towards forming symbiotic associations with nitrogen- fixing bacteria and nodulatioN.
Biosafety measures 1. The transgenic plants will be grown only in the CL4 greenhouse.
2. Every panicle of the flowering plants will be bagged to prevent cross- pollination or escape of pollen.
3. Plants used in the experiment will be destroyed by incineration after use.
4. Soil together with pots that are used to grow the plants will be placed in sealed disposal plastic bags and autoclaved.
5. The seeds will be kept in sealed aluminum foil packets inside a metal container, and stored in a locked refrigerator.
Conditions for Approval N/A
Date Received 1999-08-20
Date of Approval 2000-01-19


Regulated Materials

Regulated Materials 1. GS50, GS52 and Le1 genes; 2. DB46 gene; 3. Lb gene; 4. ENOD40; 5. CHS3;
Source 1. University of Tennessee, USA; 2. University of California Davis, USA; 3. CSIRO, Canberra, Ausralia; 4. INRA/CNRS, Toulouse, France and NIAR, Tsukuba, Japan; 5. Australian National University, Canberra, Australia;
Subject Request of Dr. D.S. Brar for permit to import GS50, GS52, DB46, Le1, Lb, ENOD40, and CHS3
Requesting Party Dr. Darshan S. Brar, Chairperson, IRRI-IBC
Action of NCBP Approved for Implementation

For issuance of permit to import GS50, GS52, DB46, Le1, Lb, ENOD40, and CHS3 by the Plant Quarantine Services, Bureau of Plant Industry.