Project Information

Reference Number 19990113
Project Title The Use of Rh(phi)2DMB3 to Repair Thymine Dimers in pUC 18 Plasmids as Determined by CD or other Spectroscopic Techniques
Project Type Contained Use (Laboratory)
Status Ongoing
Name of Institution Institute of Chemistry, College of Science, UPD;
Cooperating Institution Natural Science Research Institute (NSRI)
Supervising IBC UP Diliman-IBC
Project Leader(s) Eva Marie Ratilla
Experimental Facility/Site UP Diliman, Quezon City, Philippines
Purpose / Objectives 1. To measure in vitro repair of thymine dimers using pUC 18 plasmids transformed in E. coli JM 105 cells.
2. To examine the possibility of in vivo repair of thymine dimers using pUC 18 plasmids transformed in E. coli cells.
3. To explore the possibility of using CD as a method in measuring thymine dimer repair.
Biosafety measures 1. Containment capability of laboratory
2. Sterilization procedures
3. Personnel awareness of biosafety procedures
4. Labelling/ designation of “risk” areas
5. “Biosafetiness” of equipment
6. All plant tissues and soil used in the experiment will be autoclaved and incinerated.
Conditions for Approval N/A
Date Received 1999-09-07
Date of Approval 1999-12-15


Regulated Materials

Regulated Materials N/A
Source N/A
Subject Request of Dr. Elma Llaguno for proposal endorsement
Requesting Party Dr. Elma Llaguno, Chairman, UP Diliman IBC
Action of NCBP Approved for implementation